Protein-based Chiral Columns
The Analytical Solution for an Extremely Broad Range of Compounds
Regis carries a line of protein-based chiral columns manufactured by ChromTech including the Chiral AGP (α-glycoprotein), Chiral CBH (cellobiohydrolase), and Chiral HSA (human serum albumin), in micro, analytical and semi-preparative sized columns.
- Chiral AGP is the second generation chiral selector based on the α1-acid glycoprotein (α1-AGP) as the chiral stationary phase.
- The AGP has been immobilized on spherical, 5 µm particles. The Chiral AGP column is typically used in the reversed-phase mode, where it can be used for the resolution of an extremely broad range of chiral compounds, such as amines, (primary, secondary, tertiary and quaternary ammonium), acids, esters, sulphoxides, amides, and alcohols.
- The enantioselectivity and the retention can easily be regulated by the pH of the mobile phase, the buffer concentration and the nature and concentration of the organic modifier.
- Cellobiohydrolase (CBH) is a stable enzyme which has been immobilized onto 5 µm spherical silica particles.
- The column is used in reversed-phase mode and is effective for the separation of enantiomers of basic drugs from many compound classes.
- The retention and the enantioselectivity can be regulated by changes in pH, buffer concentration and the nature and concentration of organic modifier.
- (human serum albumin) HSA has been immobilized onto 5 µm spherical silica particles. The surface chemistry of the silica and the method of immobilization provide a stable chiral separation material.
- With the Chiral human serum albumin (HSA) column, the enantiomers of many carboxylic acids and amino acids can be resolved directly, without derivatization.
- Enantioselectivity and retention can be regulated by changing the mobile phase composition, pH, buffer concentration and/or nature of the organic modifier.