A chiral HPLC-column for direct resolution of enantiomers
The CHIRAL-HSA column is based on human serum albumin (HSA). The protein has been immobilized onto spherical 5 micrometer particles. Enantiomers of preferentially acidic compounds can be resolved directly, without derivatization. CHIRAL-HSA is especially suited for the resolution of weak and strong acids, zwitterionic and nonprotolytic compounds. However, basic compounds have also been resolved.
- At pharmaceutical companies, hospitals, universities and chemical industry CHIRAL-HSA is used for the analysis of enantiomeric purity and for bioanalysis.
- The column is operated in the reversed phase mode. Phosphate buffers with addition of organic modifiers are used as mobile phases. Retention and enantioselectivity can be regulated by changing the mobile phase composition. The addition of octanoic acid is necessary when chromatographing for example arylpropionic acid derivates on CHIRAL-HS.
||1 cm x 3.0 mm i.d. guard
||10 cm x 2.0 mm i.d.
||5 cm x 2.0 mm i.d.