A chiral HPLC-column for direct resolution of enantiomers
The chiral selector in the CHIRAL-AGP phase is alpha1-acid glycoprotein (AGP). This very stable protein has been immobilized onto spherical 5 micrometer silica particles. The protein tolerates high concentrations of organic solvents, high and low pH, and high temperatures. The column is used in the reversed-phase and can be used for direct resolution of enantiomers, without derivatization. The CHIRAL-AGP column separates enantiomers of an extremely broad range of drug compounds:
amines (primary, secondary, tertiary, and quaternary)
acids (strong and weak)
nonprotolytes (amides, esters, alcohols, sulphoxides, etc.)
CHIRAL-AGP is most likely the column with the broadest applicability of all chiral columns available today.
- At pharmaceutical companies, hospitals, universities and chemical industry CHIRAL-AGP is used for the analysis of enantiomeric purity and for bioanalysis. A growing application area, due to the exceptional applicability of the phase, is isolation of pure enantiomers on semipreparative columns.
- Very simple mobile phase systems are used for the majority of the separations; buffered waterbased mobile phases with the addition of low concentrations of an organic modifier, such as 2-propanol or acetonitrile for example. The changes affect both the solute and the protein on the stationary phase. The changes affect different types of solutes in a different ways. Most of the separations (>95%) have been performed using the above described simple mobile phases. However, in some cases charged modifiers as DMOA (N,N-dimethyloctylamine), octanic acid and quaternary ammonium compounds are used to induce enantioselectivity.
||1 cm x 3.0 mm i.d. guard
||10 cm x 2.0 mm i.d.