New Cool SIELC Application

Separation of Sulfur-containing Biomolecules

Glutathione, a tripeptide prevalent in nearly all cells, plays numerous roles in
biological systems. Comprised of three amino acids - L-glutamic acid, L-cysteine, and
glycine - natural glutathione molecules are highly polar. Their retention and separation
necessitate a specialized column and/or mobile phase. We are showing a very simple
approach with UV detection that allows quick and efficient separation of most common
glutathiones. Primesep 100 column that was used in this method is one of the most popular mixed-mode columns
that provides both a strong cation exchange interaction and a reverse phase interaction. Read More ...