A chiral HPLC-column for direct resolution of enantiomers
Cellobiohydrolase (CBH) is a very stable enzyme, which has been immobilized onto 5 micrometer spherical silica particles. This is a reversed-phase column, used for the direct separation of enantiomers. The column is preferantially used for the separation of enantiomers of basic drugs from many compound classes. CHIRAL-CBH separates preferentially compounds containing one or more basic nitrogen together with one or more hydrogen accepting or hydrogen donating groups (alcohol, phenol, carbonyl, amide, ether, sulphoxide, ester etc.).
- At pharmaceutical companies, hospitals, universities and chemical industry CHIRAL-CBH is used for the analysis of enantiomeric purity and for bioanalysis. An interesting and fast growing area is isolation of pure enantiomers on semipreparative columns.
- The mobile phases are mixtures of phosphate or acetate buffers and organic solvents as 2-propanol or acetonitrile containing 50mM disodium EDTA. The retention and the enantioselectivity can be regulated by changes in pH, buffer concentration and organic modifier (nature and concentration).
|1 cm x 3.0 mm i.d. guard
|15 cm x 2.0 mm i.d.