Sielc Technologies

Primesep HPLC Columns

Novel Stationary Phases For decades, liquid chromatography stationary phase design has been dominated by the idea of elimination of multiple, or "unwanted", interactions that occur in mixed-mode separations. Reverse-phase chromatography is making the point of eliminating silanol interactions with amine-containing analytes by developing base-deactivated phases. Within the same trend, adsorption interactions are considered undesirable as opposed to steric interactions in size-exclusion chromatography, and non-ionic interactions are generally viewed as complications in ion-exchange and ion-exclusion chromatography. However, there are ways to benefit from multiple interactions on the stationary phase. SIELC Technologies introduces Primesep® HPLC columns, specifically designed for mixed-mode separations and capable of separating a tremendous range of compounds by different separation modes based only upon mobile phase selection. MORE Currently, SIELC Technologies offers the following types of silica-based mixed-mode phases.
 
Primesep Phases:
 
Columns with negatively charged functional groups.
  • Primesep A
    • Strongest acidic phase
    • Strong acidic phase
  • Primesep 200
    • Medium acidic phase
Columns with positively charged functional groups.
  • Primesep B
    • Strong basic column for use with TFA, phosphoric or perchloric acid solutions in a mobile phase.
  • Primesep B2
    • Weak basic column for ammonium acetate buffered solution.
 
Columns with unique unparallel selectivity.
  • Primesep C
    • Combined reverse-phase and complex formation properties for interaction with amines, sulfonium, phosphonium and metal ions.
 
Three interactions within one column:
reverse phase
pi-pi
strong cation exchange
  • Primesep P
    • Choose for separation of structural isomers of aromatic compounds.