Obelisc HPLC Columns
Obelisc™ HPLC columns are another, innovative line from SIELC Technologies, the inventors of Primesep. With multiple patents pending, Obelisc columns are the first commercially available columns with Liquid Separation Cell technology ( LiSC ™). Obelisc R, with reversed-phase characteristics, and Obelisc N, with normal-phase characteristics, separate polar and non-polar compounds using multiple separation mechanisms.
This cost effective duo replaces multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction LIquid Chromatography) columns. Method development is simplified by using only two Obelisc columns and a few simple mass spec or low UV (<220 nm) compatible mobile phases. Obelisc R and Obelisc N differ in the type and proximity of their charged groups and the hydrophobicity of their long chains. Obelisc R has cationic groups close to the silica surface separated from anionic groups by a hydrophobic chain. Obelisc N has anions close to the surface separated from cationic groups by a hydrophilic chain.