Ion Pair Reagents
Ultra pure Ion pair reagents and buffers
When used with common hydrophobic HPLC phases in the reversed-phase mode, ion pair reagents can be used to selectively increase the retention of charged analytes (Figure 2).
For in-depth information, see:
Regis manufactures both ultrapure anionic Sulfonate (S-series) and cationic Quaternary Amine (Q-Series) ion pair concentrates in the following alkyl chain Iengths: pentyl, hexyl, heptyl, octyl, and dodecyl. Alkyl chains are represented by cardinal numbers in the naming of our products, i.e., 5, 6, 7, 8, and 12.
Although ion exchange chromatography has become a popular mode of separation, it is not useful in aIl situations. The advantages of ion pair chromatography over ion exchange chromatography are:
Simple preparation of buffers Wide choice of carbon chain Iengths for improved retention and separation
Signiscantly reduced separation time
Simultaneous separation of both ionized and nonionized solutes
Highly reproducible results Improved peak shape