Regis Technologies
Ion Pair Reagents
Ultra pure Ion pair reagents and buffers
Figure 1
When used with common hydrophobic HPLC phases in the reversed-phase mode, ion pair reagents can be used to selectively increase the retention of charged analytes (Figure 2).
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Regis manufactures both ultrapure anionic Sulfonate (S-series) and cationic Quaternary Amine (Q-Series) ion pair concentrates in the following alkyl chain Iengths: pentyl, hexyl, heptyl, octyl, and dodecyl. Alkyl chains are represented by cardinal numbers in the naming of our products, i.e., 5, 6, 7, 8, and 12.
Although ion exchange chromatography has become a popular mode of separation, it is not useful in aIl situations. The advantages of ion pair chromatography over ion exchange chromatography are:
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Simple preparation of buffers Wide choice of carbon chain Iengths for improved retention and separation
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Signiscantly reduced separation time
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Simultaneous separation of both ionized and nonionized solutes
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Highly reproducible results Improved peak shape