Sepax Technologies, Inc.
Biological Separation Solution
Reversed Phase Columns
Proteomix® RP packings are porous polystyrene divinylbenzene (PS/DVB) polymer resins with narrow-dispersed particle size distribution. The base matrix of the resin is made of highly cross-linked PS/DVB which functions as reversed phase for chromatographic separation. The phase structure is phenyl and substituted phenyl functional groups that enable hydrophobic interaction. These highly rigid resins are provided with the particle size selection of 5 and 10 µm. In comparison to silica based reversed phase media, Proteomix® RP phases have advantages for biomolecule separations with wide pH range (1-14) and high thermal stability. At various column temperatures, the Proteomix® RP phases offer special selectivity, high resolution separation with online mass spec analysis capability for proteins such as mAb (monoclonal antibody), ADC (antibody drug conjugate) and related protein fragments.
| Resin Matrix | Spherical, highly cross-linked PS/DVB |
| Pore size | 300, 500 and 1000 Å |
| Particle size | 5 and 10 µm |
| Phase Structure | Phenyl and substituted phenyl group |
| Separation Mechanism | Hydrophobic interaction |
| pH Stability | 1-14 |
| Operating Temperature | Up to 80 °C |
| Operating Pressure | Up to 100 bar (at 80 °C ) |
| Mobile Phase Compatibility | Compatible with aqueous solution, a mixture of water and acetonitrile, acetone, methanol, or THF |