Poly LC Inc.

HPLC Supplies for the Life Science

PolyHYDROXYETHYL A™ - for Hydrophilic Interaction and Size Exclusion HPLC -

This neutral, polar material was developed specifically for Hydrophilic Interaction Chromatography (HILIC). HILIC is a variant of normal phase chromatography that is performed with a polar stationary phase and a partly aqueous mobile phase. This permits normal-phase separations of peptides, nucleic acids, carbohydrates, some proteins, and polar solutes in general. In comparison with other materials used for HILIC, PolyHYDROXYETHYL A™ affords sharper peaks and better selectivity and recovery. Its great polarity means less organic solvent is needed to get retention.
Use PolyHYDROXYETHYL A™ for:
  1. Analysis of polar small molecules for metabolomics or analysis via HILIC-MS/MS of specific small molecules (amino acids; methotrexate) in plasma or crude extracts.
  2. Peptide separations and mapping involving differences in polar groups (Ser-; glycopeptides; etc.).
  3. Multidimensional purification of synthetic and natural peptides or fractionation of really complex digests.
  4. HPLC of solutes that aren't soluble in aqueous media (membrane proteins;phospholipids).
  5. Eliminating detergents, lipids, and salts from a sample.
  6. Oligonucleotides and their analogs.
  
 COLUMNS - 3 µm, 1500 Å    COLUMNS - 12 µm, 300 and 1500 Å
ITEM SIZE ITEM SIZE
051HY03-- 50 x 1.0 mm 204HY12-- 200 x 4.6 mm
101HY03-- 100 x 1.0 mm 209HY12-- 200 x 9.4 mm
052HY03-- 50 x 2.1 mm 259HY12-- 250 x 9.4 mm
102HY03-- 100 x 2.1 mm 2521HY12-- 250 x 21.0 mm
3.54HY03-- 35 x 4.6 mm 2550HY12-- 250 x 50.8 mm
054HY03-- 50 x 4.6 mm
104HY03-- 100 x 4.6 mm
109HY03-- 100 x 9.4 mm
 COLUMNS - 3 µm 300 and 1000 Å    CAPILLARIES - 5 µm, 300 and 1000Å
ITEM SIZE ITEM SIZE
051HY05-- 50 x 1.0 mm 050.15HY05-- 50 mm  x  150 µm
101HY05-- 100 x 1.0 mm 100.15HY05-- 100 mm  x  150 µm
151HY05-- 150 x 1.0 mm 150.15HY05-- 150 mm  x  150 µm
3.52HY05-- 35 x 2.1 mm 050.30HY05-- 50 mm  x  300 µm
052HY05-- 50 x 2.1 mm 100.30HY05-- 100 mm  x  300 µm
102HY05-- 100 x 2.1 mm 150.30HY05-- 150 mm  x  300 µm
202HY05-- 200 x 2.1 mm
3.54HY05-- 35 x 4.6 mm
054HY05-- 50 x 4.6 mm
104HY05-- 100 x 4.6 mm
204HY05-- 200 x 4.6 mm
109HY05-- 100 x 9.4 mm
209HY05-- 200 x 9.4 mm
259HY05-- 250 x 9.4 mm
2521HY05-- 250 x 21.0 mm       
 
FORMULATING A COMPLETE ITEM NUMBER
The item numbers in this list require additional numbers at the end to designate the pore diameter:
Numbers: -006, -01, -02, -03, -05, -10, and -15 correspond to the following pore diameters:
60 Å, 100 Å, 200 Å, 300 Å, 500 Å, 1000 Å, and 1500 Å, respectively.