PolyLC Inc.
HPLC supplies for the Life Science
PolyETHYL A™ - for Hydrophobic Interaction Chromatography (HIC) of Proteins and Peptides -
HIC materials separate proteins on the basis of hydrophobic character, as does RPC. However, HIC uses totally aqueous buffers, retaining tertiary structure and biological activity. Selectivity is generally superior to that of RPC for proteins and polypeptides large enough to have significant secondary or tertiary structure. Typically, a sample is eluted with a decreasing gradient of a salt such as a sulfate or phosphate. Proteins elute in order of increasing surface hydrophobicity. Surfactants (e.g., CHAPS; octylglucoside) can be added to the mobile phase if necessary. The relative hydrophobic character ofPolyPROPYL A™, PolyETHYL A™ and PolyMETHYL A™ is 100, 60 and 15, resp. HIC has greater sensitivity than other modes to the location of a modification or residue difference, especially (but not necessarily) if it involves a nonpolar group. Use these HIC materials for:
- Multidimensional protein purification (suggested sequence: Ion-exchange - [add salt to collected fractions] - HIC).
- Purification of polypeptides (e.g., venoms and glycopeptides).
- Characterization of antibodies.
- QC analysis of proteins differing in the polarity of a single residue or modified positional variants.
Use material with 1000- or 1500-Å pores for proteins > 20 KDa. Capacity is comparable to ion-exchange. Unless a protein is known to be unusually hydrophobic, use PolyPROPYL A™.
| COLUMNS - 3 µm, 1500 Å | COLUMNS - 12 µm, 300 and 1500 Å | |||
| ITEM | SIZE | ITEM | SIZE | |
| 051ET03-- | 50 x 1.0 mm | 204ET12-- | 200 x 4.6 mm | |
| 101ET03-- | 100 x 1.0 mm | 209ET12-- | 200 x 9.4 mm | |
| 052ET03-- | 50 x 2.1 mm | 259ET12-- | 250 x 9.4 mm | |
| 102ET03-- | 100 x 2.1 mm | 2521ET12-- | 250 x 21.0 mm | |
| 3.54ET03-- | 35 x 4.6 mm | 2550ET12-- | 250 x 50.8 mm | |
| 054ET03-- | 50 x 4.6 mm | |||
| 104ET03-- | 100 x 4.6 mm | |||
| 109ET03-- | 100 x 9.4 mm | |||
| COLUMNS - 5µm, 300 and 1000 Å |
CAPILLARIES - 5µm, 300 and 1000 Å | |||
| ITEM | SIZE | ITEM | SIZE | |
| 051ET05-- | 50 x 1.0 mm | 050.15ET05-- | 50 mm x 150 µm | |
| 101ET05-- | 100 x 1.0 mm | 100.15ET05-- | 100 mm x 150 µm | |
| 151ET05-- | 150 x 1.0 mm | 150.15ET05-- | 150 mm x 150 µm | |
| 3.52ET05-- | 35 x 2.1 mm | 050.30ET05-- | 50 mm x 300 µm | |
| 052ET05-- | 50 x 2.1 mm | 100.30ET05-- | 100 mm x 300 µm | |
| 102ET05-- | 100 x 2.1 mm | 150.30ET05-- | 150 mm x 300 µm | |
| 202ET05-- | 200 x 2.1 mm | |||
| 3.54ET05-- | 35 x 4.6 mm | |||
| 054ET05-- | 50 x 4.6 mm | |||
| 104ET05-- | 100 x 4.6 mm | |||
| 204ET05-- | 200 x 4.6 mm | |||
| 109ET05-- | 100 x 9.4 mm | |||
| 209ET05-- | 200 x 9.4 mm | |||
| 259ET05-- | 250 x 9.4 mm | |||
| 2521ET05-- | 250 x 21.0 mm | |||
FORMULATING A COMPLETE ITEM NUMBER
The item numbers in this list require additional numbers at the end to designate the pore diameter:
Numbers: -006, -01, -02, -03, -05, -10, and -15 correspond to the following pore diameters:
60 Å, 100 Å, 200 Å, 300 Å, 500 Å, 1000 Å, and 1500 Å, respectively.
Numbers: -006, -01, -02, -03, -05, -10, and -15 correspond to the following pore diameters:
60 Å, 100 Å, 200 Å, 300 Å, 500 Å, 1000 Å, and 1500 Å, respectively.