IAM Drugs Discovery Phase
The IAM.PC.DD2 is used to predict drug membrane permeability. The ester bonding of the DD2 packing offers more hydrophobicity than the DD phase. This material is a diacylated or double chain ester PC ligand denoted as Ester IAM.PC.DD2, and is endcapped with C10/C3 alkyl chains, as illustrated in Figure 2.
The IAM.PC.DD2 packing offers the following advantages:
The IAM.PC.DD2 offers more hydrophobicity, thus giving longer retentions to compounds not well retained on the IAM.PC.DD packing. Retentions are typically double on the IAM.PC.DD2 column than on the DD column and exhibit excellent correlation for groups of compounds.
Another distinct advantage of the IAM.PC.DD2 packing is its ability to tolerate mobile phases between pH's 7.0 and 7.5, thus resulting in longer column life under these conditions.
Excellent Correlation to Traditional Methods
Intestinal Tissue Correlation Table 1 shows that drug permeability predicted by inverted rat intestines correlates well to drug retention factors, k'IAM, measured on both the DD and DD2 columns.
|Table 1. Correlating Drug Partitioning into IAM With Rat Intestinal Drug Absorption|
|Sample||% Absorption of Inverted Rat Intestine||
|r (correlation factor)||0.8088||0.8025|
|Product||Particle Size||Column Dimensions||Code|
|IAM.PC.DD2||10 µm, 300Å||10 cm x 4.6 mm i.d.||774011|
|IAM.PC.DD2||10 µm, 300Å||15 cm x 4.6 mm i.d.||774014|
|IAM.PC.DD2||10 µm, 300Å||3 cm x 4.6 mm i.d.||774010|
|IAM.PC.DD2 Guard Cartridges**||10 µm, 300Å||1 cm x 3.0 mm i.d.||774013|
|IAM.PC.DD2 Guard Kit*||10 µm, 300Å||1 cm x 3.0 mm i.d.||774012|