Regis Technologies

The IAM.PC.DD2 is used to predict drug membrane permeability. The ester bonding of the DD2 packing offers more hydrophobicity than the DD phase. This material is a diacylated or double chain ester PC ligand denoted as Ester IAM.PC.DD2, and is endcapped with C10/C3 alkyl chains, as illustrated in Figure 2.

 

The IAM.PC.DD2 packing offers the following advantages:

 

More Hydrophobic

The IAM.PC.DD2 offers more hydrophobicity, thus giving longer retentions to compounds not well retained on the IAM.PC.DD packing. Retentions are typically double on the IAM.PC.DD2 column than on the DD column and exhibit excellent correlation for groups of compounds.

 

Greater Stability

Another distinct advantage of the IAM.PC.DD2 packing is its ability to tolerate mobile phases between pH's 7.0 and 7.5, thus resulting in longer column life under these conditions.

 

Excellent Correlation to Traditional Methods

Intestinal Tissue Correlation Table 1 shows that drug permeability predicted by inverted rat intestines correlates well to drug retention factors, k'IAM, measured on both the DD and DD2 columns.

 

Table 1. Correlating Drug Partitioning into IAM With Rat Intestinal Drug Absorption
Sample	% Absorption of Inverted Rat Intestine	(k') IAM.PC.DD	(k') IAM.PC.DD2
m-nitroaniline	77	3.875	10.838
p-nitroanaline	68	8.576	16.086
salicylic acid	60	1.936	6.963
p-toluidine	59	1.554	4.546
aniline	54	0.811	2.069
m-nitrobenzoic acid	53	1.213	4.403
phenol	51	2.965	6.544
benzoic acid	51	0.531	2.088
acetanilide	42	1.797	5.096
antipyrine	32	0.685	3.350
theophylline	29	0.583	1.478
acetylsalicylic acis	20	0.197	0.931
r (correlation factor)		0.8088	0.8025

  

Product	Particle Size	Column Dimensions	Code
IAM.PC.DD2	10 µm, 300Å	10 cm x 4.6 mm i.d.	774011
IAM.PC.DD2	10 µm, 300Å	15 cm x 4.6 mm i.d.	774014
IAM.PC.DD2	10 µm, 300Å	3 cm x 4.6 mm i.d.	774010
IAM.PC.DD2 Guard Cartridges**	10 µm, 300Å	1 cm x 3.0 mm i.d.	774013
IAM.PC.DD2 Guard Kit*	10 µm, 300Å	1 cm x 3.0 mm i.d.	774012