Sepax Technologies, Inc.
Biological Separation Solution
Sepax Antibodix Columns
Description
Antibodix columns are specially designed for high resolution, high efficiency and high recovery separations of antibodies. The packing support is composed of a rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) non-porous bead. The non-porous resin has particle size of 1.7, 3, 5 and 10 µm. The PS/DVB resin surface is grafted with a highly hydrophilic, neutral polymer thin layer with the thickness in the range of nanometer. On the top of the hydrophilic layer, weak cation-exchange functional groups are attached via a proprietary chemistry, resulting in high capacity ion-exchange layer.
Chromatogram of a protein mixture separated by Antibodix NP5, 7.8×50mm
|
Conditions:
Column: Antibodix NP5 (5 µm, 7.8×50 mm) Mobile phase: A: 10 mM phosphate buffer, pH 6 Mobile Phase B: A + 1.0 M NaCl
Gradient: 10-45%B (21 min) Flow rate: 1.5 mL/min Temperature: Ambient Injection volume: 10 µL Sample: 1. Cytochrome C 2. Lysozyme
3. Ribonuclease A
|
Packing:
Support: High cross-linked, spherical PS/DVB beads
Particle size: 1.7, 3, 5 and 10 µm
Pore size: non-porous
Phase structure: weak cation exchanger with carboxylate functional groups chemically bonded and hydrophilic
Particle size: 1.7, 3, 5 and 10 µm
Pore size: non-porous
Phase structure: weak cation exchanger with carboxylate functional groups chemically bonded and hydrophilic