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  • Fresh of the Press News
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  • Chromatography
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  • Terms and conditions
  • Starpure Catalogue 2024

Sepax Technologies, Inc.

Biological Separation Solution

Sepax Antibodix Columns

 
Description
Antibodix columns are specially designed for high resolution, high efficiency and high recovery separations of antibodies. The packing support is composed of a rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) non-porous bead. The non-porous resin has particle size of 1.7, 3, 5 and 10 µm. The PS/DVB resin surface is grafted with a highly hydrophilic, neutral polymer thin layer with the thickness in the range of nanometer. On the top of the hydrophilic layer, weak cation-exchange functional groups are attached via a proprietary chemistry, resulting in high capacity ion-exchange layer.
 
Chromatogram of a protein mixture separated by Antibodix NP5, 7.8×50mm

 
Conditions:
Column: Antibodix NP5 (5 µm, 7.8×50 mm)
Mobile phase: A: 10 mM phosphate buffer, pH 6
Mobile Phase B: A + 1.0 M NaCl
Gradient: 10-45%B (21 min)
Flow rate: 1.5 mL/min
Temperature: Ambient
Injection volume: 10 µL
Sample: 1. Cytochrome C
           2. Lysozyme
           3. Ribonuclease A

Packing:
Support: High cross-linked, spherical PS/DVB beads
Particle size: 1.7, 3, 5 and 10 µm
Pore size: non-porous
Phase structure: weak cation exchanger with carboxylate functional groups chemically bonded and hydrophilic
 
Available Columns
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