HELIX CHROMATOGRAPHY

 

 

Amaze TR is a tri-modal reversed-phase cation- and anion-exchange column. It contains a C18 carbon chain, and acidic and basic groups on the surface. All functionalities are assembled in one molecule which guarantees robust selectivity and reproducibility. The column can be used in any organic solvent – including alcohols – without the danger of esterification of acidic group on the surface. It is compatible with 100%-organic and 100%-aqueous mobile phase due to multiple polar embedded groups, and resists a collapse of stationary phase in highly aqueous mobile phase. Its carefully designed stationary phase has a high density of ligand, providing a great capacity for neutral, acidic and basic compounds.

 

Amaze QR is a quaternary-modal reversed-phase column. It contains hydrophobic chain, aromatic fragments, and acidic and basic groups on the surface. All functionalities are carried within one ligand which is attached to the surface. The column is designed to separate a wide range of compounds within a single run. Compounds are retained and separated by a combination of reversed-phase, pi-pi, cation- and anion-exchange mechanisms. Retention time and selectivity can be adjusted by using various organic solvents (acetonitrile vs. methanol), buffer pH and buffer concentration. Multi-modal interaction allow to separate compounds that differ even slightly in chemical and physical properties. The synergy of multiple interaction makes much greater difference than single mode separations. All ingredients of mobile-phase composition are important in finding right conditions for toughest separations.

 

Amaze TH is tri-modal HILIC and cation- and anion-exchange column designed to separate polar neutral, polar acidic, polar basic, hydrophobic acidic and hydrophobic basic compounds in one run. All functionalities are carried within one ligand which is attached to the surface. The strength of basic and acidic groups can be adjusted by the pH of the mobile phase, allowing to retain compounds even with weak ionizable groups. Highly polar and ionizable groups on the surface allow HILIC separation with lower concentration of ACN, which helps to significantly increase capacity of the column and loadability of compounds during analysis. Retention time for compounds can be adjusted by amount of organic, buffer pH and buffer concentration. Nature of the buffer can be used in some cases to find final tuning for separation of critical pairs of compounds.

 

Amaze HD is a bi-modal column designed to retain compounds by a combination of cation-exchange and hydrogen bonding mechanisms. Column can be used in normal, HILIC or cation-exchange modes, depending on the mobile phase composition. Column is fully compatible with all organic solvents and with 100% water. The column offers alternative selectivity to traditional reversed-phase and HILIC columns. The surface contains polymeric ligand with strong hydrogen donor groups as well as strongly acidic functionality.

 

Amaze HA is a bi-modal column designed to retain compounds by a combination of anion-exchange and hydrogen bonding mechanisms. Column can be used in normal, HILIC or anion-exchange modes depending on the mobile phase composition. Column is fully compatible with all organic solvents and 100% water. The column offers alternative selectivity to traditional reversed-phase and HILIC columns. The surface contains polymeric ligand with strong hydrogen acceptor groups as well as basic functionality.