Regis Technologies

GC Derivatization Reagents

Alkylation Reagent

3.0 N HCl in n-Butanol

3N HCl in n-Butanol is an Alkylation Reagent for GC Derivatization and is required for newborn screening for metabolic disorders. Neonatal screening, which has become a standard procedure in many countries, measures amino acids and acylcarnitines from a single drop of blood. Blood concentration of one or several of these compounds is either abnormally high or low in a variety of metabolic disorders in newborns. Derivatization with 3N HCl in n-Butanol ensures butylation of the carboxyl acid group of the analyte and formation of butyl ester, which forces ionization or makes charging of the analytes more efficient. Although direct analysis of extracted acycarnitine without derivatization is possible, according to different reports, butylesterification is superior with regard to sensitivity and specificity.

3 N HCl in n-Butanol from Regis Technologies is manufactured under cGMP protocols to assure highest quality and lot-to-lot consistency for this reagent. Each lot is tested by tandem mass spectrometry to ensure absence of contaminants which may interfere with analysis.
 
Physical and Chemical Properties
  • Suggested Storage Conditions: 2-8ºC
  • Use: Alkylation Reagent for GC
  • Appearance: Colorless to light yellow liquid
  • BP: 117-118ºC (242-243ºF) (BuOH)
  • MF: HCl + C4H10O
  • FW: 36.46 g/mol (HCl); 74.12 g/mol (BuOH)
  • Specific Gravity (H2O = 1.0): 0.890
 
3.0 N HCl in n-Butanol; 3N Hydrochloric Acid in n-Butanol
CAS [7647-01-0 (Hydrochloric Acid); 71-36-3 (n-Butanol)]
 
 
Product
Size
Regis Product #
3.0 N HCl in n-Butanol
 4 x 25 mL 201007
3.0 N HCl in n-Butanol
 100 mL 201009