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Sepax Technologies

Biological Separation Solution

Unix™ Columns

Description
Utilizing proprietary surface technologies, Unix SEC-300 phase is made of uniform, hydrophilic, and neutral nanometer thick films chemically bonded to high purity and mechanically stabilized silica with the particle size of 1.8 µm. The combination of small particle size and large pore volume of Unix SEC-300 renders the highest separation efficiency and resolution of analytes. The well-controlled surface chemistry results in excellent lot-to-lot reproducibility. Our unique bonding chemistry, coupled with the maximized bonding density, allows Unix SEC-300 to provide high stability and negligible non-specific interactions. Typical applications for Unix SEC-300 columns include separation and analysis of biological molecules and water-soluble polymers.
Phase
UnixTM SEC-300
Material Neutral, hydrophilic film bonded silica
Average Particle size
1.8 µm
Pore size
300 Å
Protein MW range (native)
5,000 - 1,250,000 Da
pH stability 2.0 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
 Backpressure for 4.6×150 mm (0.35 mL/min) ~1700 psi
Maximum backpressure
~4500 psi
Salt concentration range
20 mM - 2.0 M
Maximum temperature (°C)
~ 80°C
Mobile phase compatibility
Aqueous and organic
 
Order Information
Part Number Particle Size Pore Size ID×Length
211300-4615 1.8 µm 300 Å 4.6×150mm
211300-4630 1.8 µm 300 Å 4.6×300mm
 
Coupler
P/N: 102002-COUPLER
One-piece PEEK coupler, recommended to connect columns in series.

Precolumn Filter (0.5 µm PEEK frit)
P/N: 102000-P356
PEEK Refill Frits (For Precolumn Filter)
P/N: 102001-P356, 0.5 µm, 5 units/pk

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