Macherey-Nagel GmbH
Spherical bulk silica packings for HPLC
NUCLEODUR® is a fully synthetical type B silica (silica of 3rd generation) offering highly advanced physical properties:
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Totally spherical particle shape
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Outstanding surface microstructure
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High pressure stability
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Low metal content
NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. It is the result of MACHEREY-NAGEL's pioneering research in chromatography for more than 40 years.
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Summary of available NUCLEODUR® bulk packings
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Phase
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Modification
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% C
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pH range
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Sizes [µm]
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USP
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C18 HTec
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Octadecyl, high capacity, high density, endcapped
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18
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1–11
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5 · 7 · 10
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L1
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C18 ec
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Octadecyl, medium density, endcapped
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17.5
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1–9
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10 · 12 · 16
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20 · 30 · 50
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L1
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SiOH
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Unmodified
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–
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2–8
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10 · 12 · 16
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20 · 30 · 50
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L3
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NUCLEOSIL® standard silica phases for HPLC
NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure
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Wide acceptance as routine packings for very different fields of chromatography
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One of the first spherical silicas used in HPLC
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Developed in the early seventies, it became a world-renowned HPLC packing
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Still found in many analytical and preparative applications
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An absolutely reliable choice in HPLC
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The largest variety of modified HPLC silicas available on the market
| Summary of available NUCLEOSIL® phases | ||
| Phase | Modification | Formula |
| RP phases | ||
| NUCLEOSIL® C18 | medium density octadecyl, endcapped | -(CH2)17-CH3 |
| NUCLEOSIL® C18 AB | crosslinked octadecyl, endcapped, high steric selectivity | -(CH2)17-CH3 |
| NUCLEOSIL® C18 HD | high density octadecyl, endcapped | -(CH2)17-CH3 |
| NUCLEOSIL® C18 Nautilus | hydrophilic octadecyl, embedded polar group, endcapped | -(CH2)17-CH3 |
| NUCLEOSIL® Protect I | special RP phase with protective polar group, endcapped | |
| NUCLEOSIL® C8 ec | medium density octyl, endcapped | -(CH2)7-CH3 |
| NUCLEOSIL® C8 | standard octyl, not endcapped | -(CH2)7-CH3 |
| NUCLEOSIL® C8 HD | high density octyl, endcapped | -(CH2)7-CH3 |
| NUCLEOSIL® C4 | medium density butyl, endcapped | -(CH2)3-CH3 |
| NUCLEOSIL® C2 | dimethyl | -(CH3)2 |
| NUCLEOSIL® C6H5 | standard phenyl, not endcapped | -(CH2)3-C6H5 |
| Polar phases | ||
| NUCLEOSIL® CN | cyanopropyl (nitrile) | -(CH2)3-CN |
| NUCLEOSIL® OH | diol (dihydroxypropyl) | -(CH2)3-O-CH2-CH(OH)-CH2OH |
| NUCLEOSIL® NH2 | aminopropyl | -(CH2)3-NH2 |
| NUCLEOSIL® N(CH3)2 | dimethylamino | -(CH2)3-N(CH3)2 |
| NUCLEOSIL® SA | benzenesulfonic acid, strongly acidic cation exchanger (SCX) | -(CH2)3-C6H4-SO3Na |
| NUCLEOSIL® SB | quaternary ammonium groups, strongly basic anion exchanger (SAX) | -(CH2)3-C6H4-CH2-N+(CH3)3Cl– |
| NUCLEOSIL® SiOH | unmodified | (SiO2)n |