Macherey-Nagel GmbH
NUCLEOSHELL® core-shell silica phases for HPLC
Core-shell technology:
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Solid core of silicon dioxide,
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homogeneous shell of porous silica
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Highest efficiency compared to traditional totally porous materials
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Pore size 90 Å; particle size 2.7 µm (core 1.7 µm); specific surface 130 m2/g
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Lower back pressure also allows use on conventional LC systems
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Pressure stability up to 600 bar
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Benefits of core-shell technology
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Core-shell particles vs.
totally porous silica gel |
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Short diffusion paths
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Rapid mass transfer (C term of the Van Deemter equation)
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High flow velocity without peak broadening for fast LC
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Narrow particle size distribution (d90/d10 ~ 1.1)
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Stable packing
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High heat transfer
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Minimized influence of frictional heat
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Column efficiency of NUCLEOSHELL® ~ 250000/m (HETP ~ 4 µm)
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| Resolution Rs as function of particle size | ||
| Columns: | 50 x 4 mm NUCLEOSHELL® RP 18, 2.7 µm NUCLEODUR® C18 Gravity, 3 µm NUCLEODUR® C18 Gravity, 1.8 µm |
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| Eluent: Flow rate: Temperature: Detection: |
acetonitrile – water (60:40, v/v) 1 mL/min 25 °C UV, 254 nm |
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Peaks: 1. Naphthalene 2. Ethylbenzene |
Better resolution at lower back pressure and shorter retention tim |
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