Fast Micro-Equilibrium Dialyzer

The Fast Micro-Equilibrium DIALYZER is a unique equilibrium dialysis chamber for small samples (25 to 1500 µL) and is simple to use. Two chambers of equivalent volume are joined together with a membrane between them, as shown. Equal volumes of sample and buffer are loaded into respective chambers (the sample chamber and the assay chamber) and sealed by threaded solid caps. The DIALYZER assembly is rotated along the membrane axis from time to time. The entire system can also be placed in a thermostat for temperature controlled dialysis. When equilibrium has been reached the chambers can be opened at each end to extract the sample for analysis. When the ligand is free in solution it can readily pass through the membrane, but when complexed, it is too large and is retained by the membrane. The Fast Micro-Equilibrium DIALYZER uses membranes and chambers with high surface area to sample volume ratios. High ratio and short diffusion distances provide 2 to 5 times faster equilibration.
Each DIALYZER includes two chambers (body plus link) and two solid caps.

 

Advantage Easy to use Leak-proof Re-usable Available for a range of sample sizes Faster equiliboration High membrane area/samplevolume ratio Membranes available with MWCO’sto suit almost any application Made of Teflon – totally inert High sample recovery Low protein binding Autoclaveable

Applications Protein binding assays Protein-drug binding assays Receptor binding assays Ligand binding assays Protein-protein interations Protein-DNA interactions

Volume	25 µL	50 µL	100 µL	250 µL	500 µL	1000 µL	1500 µL
Qty. of 1	SP1 7416-251D	SP1 7416-501D	SP1 7416-1001D	SP1 7416-2501D	SP1 7416-5001D	SP1 7416-10001D	SP1 7416-15001D
Qty. of 5	SP1 7416-255D	SP1 7416-505D	SP1 7416-1005D	SP1 7416-2505D	SP1 7416-5005D	SP1 7416-10005D	SP1 7416-15005D